Development and Validation of a Reversed-Phase Liquid Chromatographic Method for Analysis of Theophylline in Human Plasma

1999 ◽  
Vol 22 (2) ◽  
pp. 131-132 ◽  
Author(s):  
Jiovanna Contreras ◽  
Elio Ontivero ◽  
Raysa González ◽  
Mireye López ◽  
David Marrero
Keyword(s):  
Human Plasma ◽  
Chromatographic Method ◽  
Reversed Phase ◽  
Liquid Chromatographic Method ◽  
Phase Liquid ◽  
Development And Validation ◽  
Liquid Chromatographic

scholarly journals Development and Validation of a High-Performance Liquid Chromatographic Method for the Determination of Cinitapride in Human Plasma

2018 ◽  
Vol 2018 ◽  
pp. 1-5
Author(s):  
Boovizhikannan Thangabalan ◽  
Getu Kahsay ◽  
Tadele Eticha
Keyword(s):  
Human Plasma ◽  
High Performance ◽  
Chromatographic Method ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Liquid Chromatographic Method ◽  
Us Fda ◽  
Development And Validation ◽  
Liquid Chromatographic

A precise and reliable reversed-phase high-performance liquid chromatographic method with ultraviolet detection was developed and validated to determine cinitapride in human plasma. After liquid-liquid extraction, chromatographic separation was achieved on a Nucleosil C18 (25 cm × 4.6 mm, 5 µm) column with an isocratic elution consisting of 10 mM ammonium acetate (pH 5.2), methanol, and acetonitrile, 40 : 50 : 10, v/v/v. The developed method was validated as per US FDA guidelines for its linearity, selectivity, sensitivity, precision, accuracy, and stability. Satisfactory findings were obtained from the validation studies. The linearity range of the method was 1 to 35 ng/mL while the extraction recovery of cinitapride in human plasma was more than 86%. The percent coefficient of variation of both intraday and interday precision was ≤7.1%.

scholarly journals Development and Validation of Liquid Chromatographic Method for Estimation of Ibuprofen and Famotidine in Combined Dosage Form

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Dimal A. Shah ◽  
Dixita J. Suthar ◽  
Sunil L. Baldania ◽  
Usman K. Chhalotiya ◽  
Kashyap K. Bhatt
Keyword(s):  
Mobile Phase ◽  
Dosage Form ◽  
Chromatographic Method ◽  
Reversed Phase ◽  
Assay Method ◽  
Liquid Chromatographic Method ◽  
Phase Liquid ◽  
Development And Validation ◽  
Liquid Chromatographic

An isocratic, reversed phase-liquid-chromatographic assay method was developed for the quantitative determination of ibuprofen and famotidine in combined-dosage form. A Brownlee C18, 5 μm column with mobile phase containing water : methanol : acetonitrile (30 : 60 : 10, v/v/v) was used. The flow rate was 1.0 mL/min, and effluents were monitored at 264 nm. The retention times of ibuprofen and famotidine were 4.9 min and 6.8 min, respectively. The linearity for ibuprofen and famotidine was in the range of 2–20 μg/mL and 0.1–10 μg/mL, respectively. The proposed method was validated with respect to linearity, accuracy, precision, specificity, and robustness. The method was successfully applied to the estimation of ibuprofen and famotidine in combined dosage form.

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